Complementary DNA Sequence of an Adenylate Translocator from Arabidopsis thaliana

کلن کردن ژنRGL2 از DNA ژنومی آرابیدوپسیس (Arabidopsis thaliana)

یکی از ژنهایی که در سنتز جیبرلین در گیاه شناخته شده و موقعیت آنها در ژنوم آرابیدپسیس مشخص شده است، RGL2 می‌باشد که در این بررسی کلن گردید. با استخراج DNA از آرابیدوپسیس، اکوتیپ کلمبیا و استفاده از آغازگرهای اختصاصی که بر اساس توالی ژن مورد نظر ساخته شده بودند، این ژن تکثیر شد. پلاسمید  PET-32a (+)و ژن تکثیر شده با استفاده از دو آنزیم برشی خاص به نامهای Nco I و Sal I برش داده شدند. پس از اتصال ژ...

H-Protein of the Glycine Decarboxylase Multienzyme Complex : Complementary DNA Encoding the Protein from Arabidopsis thaliana.

High-throughput generation of sequence indexes from T-DNA mutagenized Arabidopsis thaliana lines.

A pipeline has been created for the characterization of Arabidopsis thaliana mutants by generating flanking sequence tags (FSTs) and optimized for economic, high-throughput production. The GABI-Kat collection of T-DNA mutagenized A. thaliana plants was used as a source of independent transgenic lines. The pipeline included robotized extraction of genomic DNA in a 96-well format, an adapter-liga...

Nucleotide sequence of an Arabidopsis thaliana Lhcb4 gene.

PSII has a number of Chl a l b light-harvesting proteins (Green et al., 1991), including two polypeptides closely associated with the PSII core complex (Camm and Green, 1989). These two polypeptides were originally isolated together in one ’green band” named CP29 (White and Green, 1987; Green, 1988). Modified solubilization techniques were used later to resolve the complex into two Chl protein ...

Characterization of an Arabidopsis thaliana DNA hypomethylation mutant

We have recently isolated two Arabidopsis thaliana DNA hypomethylation mutations, identifying the DDM1 locus, that cause a 70% reduction in genomic 5-methylcytosine levels [1]. Here we describe further phenotypic and biochemical characterization of the ddm1 mutants. ddm1/ddm1 homozygotes exhibited altered leaf shape, increased cauline leaf number, and a delay in the onset of flowering when comp...